We hypothesize that some combination of peptides will yield structures that bind to different faces of the protein, so systematically testing all 1,140 possible trivalent combinations will allow us to find nano-synbodies that bind with high affinity and specificity. These peptides will be synthesized with single-stranded DNA handles, allowing them to be positioned on a DNA nanoscaffold bearing complementary handles. For the bottom-up approach, we will use a peptide array to discover 20 low-affinity peptides for a protein target. Here, we will develop antibody mimics from both top-down and bottom-up approaches.
Antibodies achieve their exquisite targeting abilities by using a protein scaffold to position three variable peptide loops in a confined nanoscale volume. We propose to create “nano-synbodies”: synthetic nanostructures that mimic antibodies in their ability to bind a target with high affinity and specificity.
Nicholas Stephanopoulos 1, Chris Diehnelt 2, and Sarah Stabenfeldt 3ġSchool of Molecular Sciences & the Biodesign Center for Molecular Design and Biomimetics, Arizona State University, Tempe, AZĢBiodesign Center for Innovations in Medicine, Arizona State University, Tempe, AZģSchool for Biological and Health Systems Engineering, Arizona State University, Tempe, AZ Synthetic antibodies from DNA-peptide hybrid nanostructures The investigators will combine their expertise in functional morphology, additive manufacturing, and artistic and engineering design to abstract design principles from insect wing venation patterns, and use Additive Manufacturing to identify those that lead to improvements in resilience of engineering structures. Within species, however, these patterns are so consistent that they are reliable taxonomic and phylogenetic characters, suggesting convergence on a functionally favorable form. The inspiration for this work comes from the venation of insect wings, which survive a wide range of loading conditions and demonstrate large diversity in patterns across species with varying local asymmetries. One such paradigm shift that we seek to explore is the use of asymmetry in constructing cellular materials, and how this may benefit overall structural integrity. Proliferation of cellular materials in engineering structures is likely to require a step-change in design thinking. A key challenge, however, is the high uncertainty of the integrity of these materials due to their heterogeneous structure. Fulton Schools of Engineering, Arizona State University, Mesa, AZĢSchool of Art, Herberger Institute for Design and the Arts, Arizona State University, Tempe, AZģSchool of Mathematical & Natural Sciences, Arizona State University, Glendale, AZĪdditive Manufacturing is increasingly enabling the incorporation of cellular structures, such as honeycombs, foams and lattices, in the manufacturing of functional parts with reduced material utilization and improved performance-to-weight ratios.
Mathematical natural sciences asu mail code pdf#
Proposals should be emailed as a PDF to Mary Kivioja awardsĪsymmetry as a design principle for improving structural resilience: A study of insect wing venation using additive manufacturingĭhruv Bhate 1, Christine Lee 2, and Lara Ferry 3ġThe Polytechnic School, Ira A. Proposal Deadline: Midnight, October 1, 2020 Preference will be given to Biomimicry Center Faculty Affiliates, as well as those that also include a faculty member from any other ASU program outside of SOLS and Engineering. Expected outcomes – Proposals should include a clear statement about the expected outcomes of the project and plans to apply for future external funding.Īll projects must be submitted by interdisciplinary teams that include at least one faculty member from the School of Life Sciences and one from the Ira A.Planned contributions of each faculty team member.Proposed activities – A clear description of the methods you will use and how you plan to allocate funds to support your project.Include any relevant citations or descriptions of your past work. Give a general overview of your proposed project and how it relates to biomimicry. Introduction and goals – Provide background context describing the problem or research area you plan to address.Project summary (200 words max) – Provide a general overview of the proposed project summaries of funded projects will be posted publicly on the Biomimicry Center website.Name and contact information of project leaders.Proposals should be organized as follows: Proposals for seed grants should not exceed 2 single-spaced pages (11pt type) including graphics and references.
0 kommentar(er)
